Obesogenic NASH models featuring all the major indicators of MASLD/NAFLD to moderate MASH/NASH, including dyslipidemia, inflammation, ballooning and robust fibrosis
Pharmacologic responses
Reference compounds (OCA, Elafibrinor, Semaglutide) in our high fat diet + modified high fat diet (HFD+mHFD) MASH/NASH mouse model match the responses of these experimental drugs in clinical trials
Histology and biomarkers
Demonstrate clinically relevant levels of disease phenotype & scores
Includes steatosis, ballooning, inflammation, mallory body and fibrosis
Metabolic profiles
Similarly altered obesity markers as does human MASH/NASH, including insulin, glucose, cholesterol, triglycerides and free fatty acid
Mechanisms of action
Drugs targeting diverse biological pathways and processes
Speed
High fat diet + modified high fat diet (HFD + mHFD) mouse model with shorter induction period between 12 – 16 weeks
High fat, high cholesterol + Fructose (HFC+F) hamster models with induction period between 8 – 12 weeks
Comprehensive
5 different MASH/NASH disease models
Multiple induction methods
Obesogenic (HFD, mHFD, AMLN)
Chemical induction (CCl4, AMLN+CCl4)
Nutrient deficient (0.1MCD)
Collaborative & Responsive
Group leaders & study directors provide rapid feedbacks and consultations to your preclinical model studies, including model selection, study design and data interpretation
MASLD/NAFLD and MASH/NASH Models & in Vitro Assays
Nutrient-deficient MASH/NASH Models
SD rat or C57bl mice fed on MCD diet for 2-3 weeks
SD rat or C57bl mice fed on 0.1MCD diet for 2-6 weeks
Chemically induced MASH/NASH Models
AMLN/HFD + CCl4 in C57bl mice, 12+4 weeks
STZ induced STAM MASH/NASH mode, 6+8 weeks
Obesogenic dietary MASH/NASH Models
HFD + modified HFD/WD in C57bl mice, 12-16 weeks
Genetic MASH/NASH Models
ob/ob mice with AMLN diet, 8-12 weeks
Hamster MASH/NASH Model
Golden Syrian hamsters
High fat & high cholesterol (HFC) plus fructose, 8-12 weeks
Fatty Liver
Liver weight
Liver Function: ALT, AST
Lipid panel (liver & serum): TC, TG, FFA
Insulin resistance: Insulin, BG
Histology: HE
Hepatic Fibrosis markers
Collagen type 1a, 3a, α-1 Procollage
SMA-a, TGF-b
Hydroxyproline
Histology Sirius Red staining
Hepatic Inflammation markers
TNF-a levels
Mononuclear cell infiltration
Oxidative Stress
Reactive oxygen species
Hydroxynonenal (HNE) level
Preclinical Models for MASH/NASH Disease
MASH/NASH Mouse and Animal Models Development and Testing
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