Biochemical Assays Overview
Biochemical assays play a critical role in drug discovery, serving as an essential first step in evaluating molecular interactions and biological activity. Typically conducted early in the drug discovery process, these assays identify promising candidates before advancing to cell-based in vitro assays, which provide further insights into their biological effects.
By measuring how drug candidates interact with receptors, enzymes or other biological targets, biochemical drug discovery assays generate precise, reproducible data for lead selection and optimization.
Our Biochemical Assay Services
BioDuro offers a comprehensive range of biochemical assays to support drug discovery at every stage. Our high-throughput screening capabilities and
custom assay development options ensure that each study is tailored to meet your research objectives.
Enzymatic Assays
BioDuro provides a diverse selection of enzymatic assays to evaluate how drug candidates interact with key enzymes. These assays help determine enzyme activation, inhibition and modulation, which are critical for developing targeted therapies.
Kinase
Phosphatase
Protease
GTPase
Oxidase
Hydrolase
Carboxylase
Nucleotidase
DNA polymerase
Phosphohydrolyase
Nicotinamide
Phosphoribosyltransferase
GPCR-Related Assays
G-protein-coupled receptors (GPCRs) are among the most common drug targets, playing major roles in cell signaling and disease mechanisms. BioDuro offers GPCR-related assays to assess whether drug candidates successfully bind to, activate or inhibit GPCRs.
³H–labeled ligand binding assay
SPA assay
Fluorescence polarization assay
Epigenetics-Related Assays
Epigenetic modifications influence gene expression and play a critical role in oncology, neurology and other disease areas. BioDuro offers a range of assays to evaluate the impact of drug candidates on epigenetic processes, helping identify potential therapeutic targets.
Writers: Histone methyltransferase, Histone acetyltransferase, Protein arginine methyltransferase (e.g. MTA-cooperative PRMT5 assays), RNA modification gene (e.g. METTL3/14 assay), Poly(ADP)-ribose polymerase: PARPs.
Erasers: Demethylase (e.g., HDACs, KDMs), Deubiquitinase (e.g. DUB).
Readers: Bromo domain containing protein binding partner assays (e.g. BRDs).
Why Choose BioDuro for Biochemical Assays?
BioDuro provides specialized expertise, advanced technology and scalable solutions to accelerate drug discovery. With a track record of delivering high-quality, reproducible data, we ensure precision throughout your research.
Comprehensive Assay Portfolio – A broad selection of in vitro biochemical assays, including enzymatic, GPCR and epigenetics-related assays, tailored to diverse research needs.
Advanced Detection Technologies – Cutting-edge methodologies, including fluorescence polarization, SPA assays, and ³H ligand-binding assays, ensure high-quality data.
Custom & Standard Assay Solutions – Choose from validated assays or collaborate with our scientists to develop tailored solutions for specific targets and pathways.
High-Throughput Screening (HTS) – Rapid, large-scale compound screening for efficient lead identification and optimization.
Industry-Leading Expertise – Work with Ph.D.-level scientists specializing in assay development, drug metabolism and pharmacology.
Global Infrastructure & Scalability – With sites and staff in the U.S. and China, we provide fast, flexible solutions for projects of all sizes.
Regulatory-Ready Research – Studies aligned with FDA, EMA and NMPA standards to support preclinical and clinical development.
Get in Touch With Us
Looking for a trusted partner in biochemical assay development?
Contact us today to discuss how BioDuro can support your research needs.
Q&A
How do BioDuro’s biochemical assays support drug development?
Our biochemical assays measure molecular interactions between drug candidates and biological targets (e.g., enzymes, receptors). These data help researchers determine binding affinity, enzyme activity and mechanism of action, guiding decision-making in early drug discovery and lead optimization.
Why are biochemical assays critical in drug discovery?
Biochemical assays are essential in drug discovery for evaluating a compound’s efficacy, specificity and toxicity before further development. They help:
Identify promising drug candidates through precise molecular interaction data
Screen for potential toxicity risks before advancing to in vitro and in vivo testing
Validate biological activity to determine whether a compound is suitable for further development
What’s the difference between biochemical and biological assays?
Biochemical assays assess molecular interactions, such as enzyme activity or receptor binding.
Biological assays, on the other hand, evaluate cellular responses to drug candidates.
Most drug discovery programs incorporate both types of assays to build a complete understanding of a compound’s activity.
How are custom biochemical assays developed?
BioDuro collaborateas clients to:
1. Define assay requirements based on drug targets and therapeutic goals.
2. Select the appropriate assay technologies for optimal sensitivity and reproducibility.
3. Develop, optimize and validate the assay with rigorous data analysis and reporting.
Home
